Orthopedic regenerative sports medicine: info@austinorthobio.com

Intra-articular injection of micronized dehydrated human amnion/chorion membrane attenuates osteoarthritis development


 

Small animal model study of the potential effect of allograft (placental/chorionic & amniotic) stem cells & growth factors on arthritis progression. Needs further study to translate to clinical use in humans.  -Kelly Cunningham, MD


 

Abstract

Introduction: Micronized dehydrated human amnion/chorion membrane (μ-dHACM) is derived from donated human placentae and has anti-inflammatory, low immunogenic and anti-fibrotic properties. The objective of this study was to quantitatively assess the efficacy of μ-dHACM as a disease modifying intervention in a rat model of osteoarthritis (OA). It was hypothesized that intra-articular injection of μ-dHACM would attenuate OA progression.

Methods: Lewis rats underwent medial meniscal transection (MMT) surgery to induce OA. Twenty four hours post-surgery, μ-dHACM or saline was injected intra-articularly into the rat joint. Naïve rats also received μ-dHACM injections. Microstructural changes in the tibial articular cartilage were assessed using equilibrium partitioning of an ionic contrast agent (EPIC-μCT) at 21 days post-surgery. The joint was also evaluated histologically and synovial fluid was analyzed for inflammatory markers at 3 and 21 days post-surgery.

Results: There was no measured baseline effect of μ-dHACM on cartilage in naïve animals. Histological staining of treated joints showed presence of μ-dHACM in the synovium along with local hypercellularity at 3 and 21 days post-surgery. In MMT animals, development of cartilage lesions at 21 days was prevented and number of partial erosions was significantly reduced by treatment with μ-dHACM. EPIC-μCT analysis quantitatively showed that μ-dHACM reduced proteoglycan loss in MMT animals.

Conclusions: μ-dHACM is rapidly sequestered in the synovial membrane following intra-articular injection and attenuates cartilage degradation in a rat OA model. These data suggest that intra-articular delivery of μ-dHACM may have a therapeutic effect on OA development.

Arthritis Research & Therapy 2014 16:R47.

Nick J Willett, Tanushree Thote, Angela SP Lin, Shamus Moran, Yazdan Raji, Sanjay Sridaran, Hazel Y Stevens, Robert E Guldberg


Disclaimer: Austin Ortho+Biologics is not affiliated with the data, content, or conclusions of this article.

Download PDF 

Location

Austin Ortho + Biologics
3003 Bee Cave Road, Suite 200
Austin, TX 78746
Phone: 512-410-0767
Fax: 512-649-7402

Office Hours

Get in touch

512-410-0767